Journal: Cell reports

Article Title: Relapse to cocaine seeking is regulated by medial habenula NR4A2/NURR1 in mice

doi: 10.1016/j.celrep.2024.113956

Figure Lengend Snippet: (A) Experimental timeline. After receiving infusion and catheter surgeries, mice self-administered cocaine for 12 days in operant conditioning chambers (D1-12). During self-administration, active lever presses resulted in a cocaine reward (8.5 μg/kg/infusion) plus a tone/light cue presentation. After 12 days of self-administration, mice experienced a 30-day homecage withdrawal period. Mice were then extinguished in a single 5-h extinction session, in which lever presses were inconsequential. Reinstatement was then induced by exposing mice to the drug-paired cues for 10 min preceding a 1-h cued reinstatement session, during which lever presses elicited cues but no cocaine rewards. Animals were sacrificed, and tissue was collected 1 h after reinstatement. (B) Schematic of Nurr2c viral delivery to MHb and expression. Protein expression, fused V5 tag (green) compared to DAPI (blue). Scale bar, 100 μm. (C) Transcript expression, BaseScope of MHb showing Nr4a2 (red) and Nurr2c (green) transcripts in GFP mice (left) and Nurr2c mice (right). (D) Cocaine self-administration followed by 30-day homecage withdrawal. Data analyzed using two-way repeated-measures ANOVA. Error bars denote SEM. GFP males n = 10, NURR2C males n = 14. (E) Five-hour extinction session. Data analyzed using two-way repeated-measures ANOVA. Error bars denote SEM. GFP males n = 10, NURR2C males n = 14. (F) Cued reinstatement. Gray circles indicate included in snRNA-seq analysis. Data analyzed using one-way ANOVA. Error bars denote SEM. **p < 0.01.

Article Snippet: Self-administration and extinction were analyzed using a two-way repeated measures ANOVA (Prism 10, GraphPad Software Inc.).

Techniques: Expressing